diff --git a/release/scripts/startup/bl_ui/properties_data_gpencil.py b/release/scripts/startup/bl_ui/properties_data_gpencil.py
index 184440a3400..b53e8c62599 100644
--- a/release/scripts/startup/bl_ui/properties_data_gpencil.py
+++ b/release/scripts/startup/bl_ui/properties_data_gpencil.py
@@ -125,16 +125,13 @@ class DATA_PT_gpencil_datapanel(Panel):
             self.draw_layers(context, layout, gpd)
 
     def draw_layers(self, context, layout, gpd):
-        userpref = context.user_preferences
-        edit = userpref.edit
-        reverse = edit.use_grease_pencil_reverse_layers
 
         row = layout.row()
 
         col = row.column()
         layer_rows = 7
         col.template_list("GPENCIL_UL_layer", "", gpd, "layers", gpd.layers, "active_index",
-                          rows=layer_rows, reverse=reverse)
+                          rows=layer_rows, reverse=True)
 
         col = row.column()
 
diff --git a/release/scripts/startup/bl_ui/space_topbar.py b/release/scripts/startup/bl_ui/space_topbar.py
index f40307b5b11..0f68b16f71a 100644
--- a/release/scripts/startup/bl_ui/space_topbar.py
+++ b/release/scripts/startup/bl_ui/space_topbar.py
@@ -352,15 +352,12 @@ class TOPBAR_PT_gpencil_layers(Panel):
             self.draw_layers(context, layout, gpd)
 
     def draw_layers(self, context, layout, gpd):
-        userpref = context.user_preferences
-        edit = userpref.edit
-        reverse = edit.use_grease_pencil_reverse_layers
         row = layout.row()
 
         col = row.column()
         layer_rows = 10
         col.template_list("GPENCIL_UL_layer", "", gpd, "layers", gpd.layers, "active_index",
-                          rows=layer_rows, reverse=reverse)
+                          rows=layer_rows, reverse=True)
 
         col = row.column()
 
diff --git a/release/scripts/startup/bl_ui/space_userpref.py b/release/scripts/startup/bl_ui/space_userpref.py
index 1516494abbc..420fa656b85 100644
--- a/release/scripts/startup/bl_ui/space_userpref.py
+++ b/release/scripts/startup/bl_ui/space_userpref.py
@@ -274,10 +274,6 @@ class USERPREF_PT_edit(Panel):
         col.prop(edit, "grease_pencil_euclidean_distance", text="Euclidean Distance")
         col.separator()
 
-        col.label(text="Grease Pencil:")
-        col.prop(edit, "use_grease_pencil_reverse_layers", text="Layers order Top-Down")
-        col.separator()
-
         col.label(text="Annotations:")
         sub = col.row()
         sub.prop(edit, "grease_pencil_default_color", text="Default Color")
diff --git a/source/blender/editors/gpencil/gpencil_data.c b/source/blender/editors/gpencil/gpencil_data.c
index 5cc5c55b3df..305aa03404b 100644
--- a/source/blender/editors/gpencil/gpencil_data.c
+++ b/source/blender/editors/gpencil/gpencil_data.c
@@ -338,8 +338,7 @@ static int gp_layer_move_exec(bContext *C, wmOperator *op)
 	bGPdata *gpd = ED_gpencil_data_get_active(C);
 	bGPDlayer *gpl = BKE_gpencil_layer_getactive(gpd);
 
-	const bool reverse = (bool)(U.gp_settings & GP_PAINT_REVERSE_LAYERS);
-	const int direction = RNA_enum_get(op->ptr, "type") * (reverse ? -1 : 1);
+	const int direction = RNA_enum_get(op->ptr, "type") * -1;
 
 	/* sanity checks */
 	if (ELEM(NULL, gpd, gpl))
@@ -1084,18 +1083,8 @@ void GPENCIL_OT_layer_isolate(wmOperatorType *ot)
 static int gp_merge_layer_exec(bContext *C, wmOperator *op)
 {
 	bGPdata *gpd = ED_gpencil_data_get_active(C);
-	const bool reverse = (bool)(U.gp_settings & GP_PAINT_REVERSE_LAYERS);
-	bGPDlayer *gpl_current = NULL;
-	bGPDlayer *gpl_next = NULL;
-
-	if (!reverse) {
-		gpl_current = BKE_gpencil_layer_getactive(gpd);
-		gpl_next = gpl_current->next;
-	}
-	else {
-		gpl_next = BKE_gpencil_layer_getactive(gpd);
-		gpl_current = gpl_next->prev;
-	}
+	bGPDlayer *gpl_next = BKE_gpencil_layer_getactive(gpd);
+	bGPDlayer *gpl_current = gpl_next->prev;
 
 	if (ELEM(NULL, gpd, gpl_current, gpl_next)) {
 		BKE_report(op->reports, RPT_ERROR, "No layers to merge");
diff --git a/source/blender/makesdna/DNA_userdef_types.h b/source/blender/makesdna/DNA_userdef_types.h
index a18ed19a306..75811ad5369 100644
--- a/source/blender/makesdna/DNA_userdef_types.h
+++ b/source/blender/makesdna/DNA_userdef_types.h
@@ -885,7 +885,6 @@ typedef enum eText_Draw_Options {
 typedef enum eGP_UserdefSettings {
 	GP_PAINT_DOSMOOTH		    = (1 << 0),
 	GP_PAINT_DOSIMPLIFY		    = (1 << 1),
-	GP_PAINT_REVERSE_LAYERS     = (1 << 2),
 } eGP_UserdefSettings;
 
 enum {
diff --git a/source/blender/makesrna/intern/rna_userdef.c b/source/blender/makesrna/intern/rna_userdef.c
index 1470681dec0..5783e9252ed 100644
--- a/source/blender/makesrna/intern/rna_userdef.c
+++ b/source/blender/makesrna/intern/rna_userdef.c
@@ -3994,17 +3994,11 @@ static void rna_def_userdef_edit(BlenderRNA *brna)
 	RNA_def_property_boolean_sdna(prop, NULL, "gp_settings", GP_PAINT_DOSIMPLIFY);
 	RNA_def_property_ui_text(prop, "Grease Pencil Simplify Stroke", "Simplify the final stroke");
 
-	prop = RNA_def_property(srna, "use_grease_pencil_reverse_layers", PROP_BOOLEAN, PROP_NONE);
-	RNA_def_property_boolean_sdna(prop, NULL, "gp_settings", GP_PAINT_REVERSE_LAYERS);
-	RNA_def_property_ui_text(prop, "Layers list Top-Down",
-		"Order the grease pencil list of layers from Top to Down");
-	
 	prop = RNA_def_property(srna, "grease_pencil_eraser_radius", PROP_INT, PROP_PIXEL);
 	RNA_def_property_int_sdna(prop, NULL, "gp_eraser");
 	RNA_def_property_range(prop, 1, 500);
 	RNA_def_property_ui_text(prop, "Grease Pencil Eraser Radius", "Radius of eraser 'brush'");
 
-
 	prop = RNA_def_property(srna, "grease_pencil_default_color", PROP_FLOAT, PROP_COLOR_GAMMA);
 	RNA_def_property_float_sdna(prop, NULL, "gpencil_new_layer_col");
 	RNA_def_property_array(prop, 4);